Idt for illumina

Idt for illumina. The Illumina DNA PCR-Free Prep delivers PCR-free sequencing-ready libraries for sensitive applications such The IDT for Illumina DNA/RNA UD Indexes Sets A-B (20027213, 20027214) 与 Illumina RNA Prep with Enrichment 和 Illumina DNA PCR-Free 试剂盒兼容 IDT for Illumina RNA Unique Dual Indexes, Ligation 试剂盒 Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20827935 Author: Ramos, Frincess Created Date: 20240215200751Z IDT for Illumina Nextera DNA UD Indexes Set D for NovaSeq, MiSeq, HiSeq 2500 and HiSeq 2000-label-sheet. Use the following sample sheet templates with IDT for Illumina Nextera DNA UD Indexes. January 30, 2025 is the last date IDT Illumina DNA/RNA UD Indexes Sets A–D (Catalog nos. IAPS. Similar to Index Sets A-D, but desalted rather than HPLC purified. Documentation. The following table shows index adapters (wells) that can be UDIs are particularly important when using instruments with patterned flow cells, such as the NovaSeq ® system from Illumina. IDT for Illumina Nextera DNA Unique Dual Indexes Set D (96 Indexes, 96 Samples) 20027216. For example, A1 = UDI0001, H1 = UDI0008, and A2 = UDI0009. Documentation, FAQs, and other support resources for the IDT for Illumina DNA/RNA UD Indexes Yes, the IDT for Illumina DNA/RNA Unique Dual (UD) Indexes can be used with the Illumina 16S Metagenomic Sequencing Library Protocol. View Online Help Mar 11, 2024. Use the Worklow A template for the HiSeq 2000, HiSeq IDT for Illumina UD Indexes. After library preparation, denature and dilute libraries to the appropriate loading concentration using the instructions for standard normalized libraries. Yes, DNA and RNA from a single sample can be analyzed with VARIANTPlex™ and FUSIONPlex™ panels to provide a genomic profile of the blood cancer. Announcements. IDT for Illumina TruSeq UD Indexes Custom Library Kit File (24 Indexes, TSV Format) ZIP(< 1 MB) Oct 17, 2017. Illumina Knowledge. This document and its contents shall not be used or distributed for any other purpose and/or Title: eCofA IDT for Illumina UMI DNA Index Anchors - 20825104 Created Date: 20240206173912Z Title: eCofA IDT for Illumina UMI DNA Index Anchors - 20896206 Created Date: 20241022175518Z Yes, DNA and RNA from a single sample can be analyzed with VARIANTPlex™ and FUSIONPlex™ panels to provide a genomic profile of the blood cancer. For information on which application use, see IDT for Illumina DNA/RNA UD Indexes Compatible Products. Tubes. The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued; the last order date is Title: eCofA IDT for Illumina DNA/RNA UD Indexes for Automation - 20813106 Author: Tabrizy, Rowshan Created Date: 20240110075651Z IDT for Illumina DNA/RNA UdIndexes, Set C Tagmentation (96 Indexes, 96 Samples) 20042666: 1: IDT for Illumina DNA/RNA UdIndexes, Set D Tagmentation (96 Indexes, 96 Samples) 20742667: Illumina Lysis Reagent Kit Box 1, Store at -25°C to -15°C. IDT for Illumina UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples) 20034701. Complete customizability. The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued; the last order date is Local Run Manager supports users in the process of planning runs, sequencing, analysis, and viewing results. Local Run Manager Files iSeq 100 and MiSeq. The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued; the last order date is When using the obsolete IDT for Illumina DNA/RNA UD Index Sets A, B, C, and D with NextSeq 1000/2000 and NovaSeq X instruments, we have observed a high percentage of 1 base pair mismatch in certain UD index pairs. Illumina Adapter Sequences The V2 indication applies only to IDT for Illumina DNA/RNA and PCR UD Indexes. 2 Next, 10 µl Illumina sequencing adapters and unique dual-index barcodes (iDT for illumina DNa/RNa UD indexes Set a, Tagmentation, catalog no. TruSeq Stranded This section provides guidelines for pooling Nextera, Illumina Prep, and Illumina PCR index kits. Illumina Adapter Sequences IDT for Illumina DNA/RNA UD Indexes are extension-based unique dual (UD) indexes with unrelated adapters for both index reads. The following tables depict plate layouts and pooling strategies for IDT for Illumina UD Indexes, which are designed for dual indexing. Illumina is improving human health by unlocking the power of the genome. The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued; the last order date is Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20835457 Created Date: 20240304201216Z Figure 2. When using the obsolete IDT for Illumina DNA/RNA UD Index Sets A, B, C, and D with NextSeq 1000/2000 and NovaSeq X instruments, we have observed a high percentage of 1 base pair mismatch in certain UD index pairs. Procedure. Nextera XT Index Kit v2 Set A Can IDT for Illumina DNA/RNA UD Indexes be used with the Illumina 16S Metagenomics Protocol? Reference Material Custom Third party Library Prep. for Element Biosciences Sequencers. IDT for Illumina –TruSeq RNA UD Indexes. Illumina today announced an expanded partnership with Integrated DNA Technologies (IDT) to develop advanced solutions for next-generation sequencing (NGS) library preparation multiplexing and a co-marketing agreement linking Illumina library prep with the IDT Exome Enrichment. Microarray. 3 The Illumina DNA Prep kit is compatible with a IDT for Illumina DNA/RNA UD Indexes, IDT for Illumina Nextera DNA UD Indexes, or Nextera DNA CD Indexes (96 Indexes, 96 Samples) Plates, Store at -25°C to -15°C. For example, the additional A base is in parentheses in the i7 adapter, as follows. IDT for Illumina UD Indexes. Whether your project requires a basic indexing solution, or a more sophisticated design for higher accuracy, we have the products and know-how to deliver the right solution. Dependingontheassay You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. Single indexing. DATE. Similar to Index Sets A-D, but desalted rather than HPLC purified Support Center / IDT for Illumina TruSeq UD Indexes Support. 26 May 2017. May 26, 2017. Instrument Administration. IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell. For index adapter sequences, refer to IDT for Illumina DNA/RNA UD Indexes Documentation. Plate layout for the Illumina DNA/RNA UD Indexes (v3) Product page and support page links for This document and its contents are proprietary to Illumina, Inc. Prepare libraries for Illumina sequencing in less than a day. This training can be delivered with select Illumina fixed panels (Exome or TruSight Cancer), Illumina custom DNA panels, or select third party DNA panels (IDT xGen Exome or Twist Human Core Exome). IDT for Illumina Nextera DNA UD Indexes Set D for NovaSeq, MiSeq, HiSeq 2500 and HiSeq 2000-label-sheet. The following figures show the plate layouts for IDT for Illumina TruSeq UD Indexes. Compatible only with IDT for Illumina RNA UD indexes, Ligation. 37% =96 wells Pass [Kit] Name IDT-Ilmn DNA-RNA UD Indexes SetA Tagmentation-RNA Enrichment Description IDT for Illumina DNA-RNA UD Indexes Set A (96 Indexes) Tagmentation to be used with Illumina RNA Prep with Enrichment IndexStrategy DualOnly ReadType Paired DefaultReadLength1 74 DefaultReadLength2 74 [Resources] Name Type Format Value Adapter Adapter string IDT for Illumina DNA/RNA UD Indexes uses 10 base pair index codes that differ from the Nextera XT and Nextera XT v2 indexes, which use eight base pair index codes. Adapter IDT for Illumina DNA/RNA UD Indexes are extension-based unique dual (UD) indexes with unrelated adapters for both index reads. The index adapter sequences are 10 bases long. IDT for Illumina UMI DNA/RNA UD Indexes Set A January 30, 2025 is the last date IDT Illumina DNA/RNA UD Indexes Sets A–D (Catalog nos. Illumina® Illumina® Reagent format Adaptors and primers were from either the IDT® for Illumina –TruSeq® DNA UD Indexes (Illumina #20022370), or the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs), NEB #E6440. 37% =96 wells Pass Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20827938 Author: Ramos, Frincess Created Date: 20240215195503Z. Adapters & Indexing Primers are a key component of NGS library preparation workflow. Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20815974 Author: Tabrizy, Rowshan Created Date: 20240111023238Z Title: eCofA IDT for Illumina DNA/RNA UD Indexes for Automation - 20828965 Created Date: 20240222160639Z You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. (Illumina), using a high output 300 cycle kit and the manufacturer's protocol. View less. Our focus on innovation has established us as a global leader in DNA sequencing and array-based technologies, serving customers in the research, clinical, and applied markets. Purchase library prep and enrichment reagents and probe panels IDT for Illumina - UMI Index Anchors: OverrideCycles,Y151;I8;U10;Y151. IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq. DNA Library Prep. Dual-index sequencing on a paired-end flow cell follows 1 of 2 workflows, depending on the system: Workflow A — The Index 2 Read is performed before Read 2 resynthesis, so the Index 2 (i5) adapter is sequenced on the forward strand. IDT for Illumina PCR Index Sets 1-4 (384 IDX) includes four PCR plates. As a global company that places high What are the sequences for the IDT and Illumina® indexes: IDT 8, IDT 10, ILMN 8, ILMN 10? These index sequences will be provided once your order has been placed. The system includes an advanced, yet accessible, cloud-based CRISPR data IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage. Plate layout for the Illumina DNA/RNA UD Indexes (v3) Product page and support page links for We recommend ordering the Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). UMI DNA Index Anchors (Plate = 20027219, Box = 20032119) + Nextera Compatible Unique Dual Index A (Sales Kit = 20027213, Plate = 20025019, Box = 20026121) View less. Amplicon Library Prep. Local Run Manager supports users in the process of planning runs, sequencing, analysis, and viewing results. / Results Applications. A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Local Run Manager software. Name. 20027213, 20027214, 20042666, and 20042667) and IDT for Illumina RNA UD Indexes Sets A–D (Catalog nos. For enhanced content portability, Illumina Cell-Free DNA Prep with Enrichment is compatible with user-supplied enrichment oligo nucleotides from Illumina or third-party vendors, including single-stranded DNA (ssDNA) from Integrated DNA Technologies (IDT) and double-stranded DNA (dsDNA) from Twist Bioscience. SIZE. After 4 PCR cycles, libraries were quantified on an Agilent ® TapeStation ® 4000. Dual indexing. 37% =96 wells Pass Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20896203 Created Date: 20241025141745Z The steps for amplicon sequencing with the Midnight Panel and the IDT Lotus DNA Library Prep Kit include cDNA synthesis, amplicon generation, fragmentation, end prep, adapter ligation, and a final PCR amplification. IDT for Illumina TruSeq UD Indexes Sample Sheet Template for Local Run Manager. Heatmaps show the percent of reads for all i5 and i7 sample index combinations in 1-, 4-, 8-, Idt for Illumina dNA/RNA Ud Indexes sets A to d allow users to generate up to 384 metagenomic libraries. IDT for Illumina RNA UD Indexes Set A, Ligation (96 indexes, 96 samples) 20040553 IDT for Illumina RNA UD Indexes Set B, Ligation (96 indexes, 96 samples) 20040554 IDT for Illumina RNA UD Indexes Set C, A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Illumina Experiment Manager (IEM) software. IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples) 20040870. Provides the nucleotide sequences that comprise Illumina oligonucleotides used in Illumina sequencing About Illumina. Single and dual indexing. We recommend using illumina Purification bead, for Illumina Sequencers. Indexes and Purification Beads sold separately. IDT scientists have tested and validated these 96 sample index sequences to Find Archer now at IDT! Illumina® sequencing platforms are not compatible with this workflow. We recommend ordering the Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). For more information, refer to Color Balance or contact Illumina Technical Support. xlsx IDT for Illumina DNA-RNA UD Indexes Set A Tagmentation-label-sheet. xlsx IDT for Illumina DNA-RNA UD Indexes Set A-D Tagmentation-label-sheet. HiSeq X. TruSeq Stranded The V2 indication applies only to IDT for Illumina DNA/RNA and PCR UD Indexes. Sign in to add to cart or see pricing. The IDT for Illumina TruSeq unique dual (UD) index adapters are arranged in the plate to enforce the recommended pairing strategy. IDT for Illumina TruSeq UD Indexes are compatible with the following library prep kits. IDT for Illumina RNA UD Indexes Set A, Ligation (96 Indizes, 96 Proben) 20040553 IDT for Illumina RNA UD Indexes Set B, Ligation (96 Indizes, 96 Proben) 20040554 IDT for Illumina RNA UD Indexes Set C, Product: IDT for Illumina®- DNA/RNA UD Indexes Set D Product number: 10016418 Illumina part number: 20084070 Batch number: 440609826 Date of manufacture: 2023-MAY-03 Expiration date: 2025-MAY-02 Analysis date: 2022-MAY-22 Test SpecificationResults Adapter QC by NGS 95% LCI* for Q30 purity ≥90. The xGen 16S Amplicon Panel v2 and the xGen ITS1 Amplicon Panel offer a reliable NGS workflow that provides coverage and NGS quality data on Illumina ® sequencing platforms. 2：Illumina DNA Prep with Enrichment, (S) Tagmentation2 试剂盒原名为Nextera Flex for Enrichment 3 ：IDT for Illumina -DNA/RNA UD Indexes Set A-D原名为IDT for Illumina Nextera DNA UD Indexes Set A-D 4 ：Illumina DNA PCR-Free Prep, Tagmentation此文库制备试剂盒仅与v1 sets A & B, and v2 sets C & D搭配进行过验证 Illumina Stranded mRNA Prep, Ligation (16 Proben) 20040532 Illumina Stranded mRNA Prep, Ligation (96 Proben) 20040534 Indizes Katalog-Nr. Learn more about multiplexing. Document # 1000000041074 v13. For index adapter sequences, see the Illumina Adapter Sequences Document. Purchase library prep and enrichment reagents and probe panels separately. Short-read sequencing technologies like those from Illumina cannot readily analyze very long DNA strands, so DNA must be fragmented into smaller, uniform pieces. Version 2 contains improvements for 8 of the 192 index oligos in plate 20022370, with no changes to the remaining 184. DNA. Purchaser is solely responsible for all IDT for Illumina RNA UD Indexes Set C Ligation (96 indexes, 96 samples) (20040555) IDT for Illumina RNA UD Indexes Set D Ligation (96 indexes, 96 samples) (20040556) Loading Concentration. Quantity. We are not permitted to share the ILMN 10 sequences pre-sale. Library Preparation. Plate layout for the Illumina DNA/RNA UD Indexes (v3) Product page and support page links for Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Purchase User documentation for the IDT for Illumina DNA/RNA UD Indexes [Kit] Name IDT-Ilmn DNA-RNA UD Indexes SetABCD Tagmentation-RNA Enrichment Description IDT for Illumina DNA-RNA UD Indexes Set A B C D (384 Indexes) Tagmentation to 2：Illumina DNA Prep with Enrichment, (S) Tagmentation2 试剂盒原名为Nextera Flex for Enrichment 3 ：IDT for Illumina -DNA/RNA UD Indexes Set A-D原名为IDT for Illumina Nextera DNA UD Indexes Set A-D 4 ：Illumina DNA PCR-Free Prep, Tagmentation此文库制备试剂盒仅与v1 sets A & B, and v2 sets C & D搭配进行过验证 IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) 20042666. xGen Universal Blockers—10 bp TS Mix is compatible with ligation-based IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage. These reagents are shipped at -25°C to -15°C. MiSeq. General iSeq 100. Illumina adapter portfolio. OverrideCycles,Y151;I8U9;I8;Y151. 5-day onsite training for the complete Illumina DNA Prep Enrichment library preparation workflow, with detailed hands-on instruction. List Price: Discounts: Illumina® Stranded Total RNA Prep, Ligation with Ribo-Zero Plus (96 Samples) 20040529. Hamilton. These include both IDT and Illumina 8- and 10-base index series, as well as the ability to upload your own index files. The IDT for Illumina UD Indexes include IDT for Illumina Nextera DNA UD Indexes, IDT for Illumina DNA/RNA UD Indexes, and IDT for Illumina PCR UD Indexes. TruSeq DNA CD Indexes. IDT for Illumina TruSeq Indexes: Up to 96-plex. These kits leverage multiplex PCR technology, enabling library construction from DNA using tiled primer pairs to target V1-V9 variable rRNA regions and the ITS1 region, each with a Local Run Manager supports users in the process of planning runs, sequencing, analysis, and viewing results. It is mission critical for us to deliver innovative, flexible, and scalable solutions to meet the needs of our customers. NextSeq 1000/2000. Index 1 (i7) Adapters We recommend ordering the Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). xGen Universal Blockers—TS Mix blocks Illumina LT (p5, p7 – 6 nt and 8 nt) and HT (i5, i7) adapters and is compatible with ligation-based library prep kits, such as Illumina TruSeq kits. HiSeq 1000/2500. The IDT for Illumina UD Illumina UD Indexes and IDT for Illumina UD Indexes: Up to 384-plex. Illumina Korea Hi Investment & Securities building 66 Yeoidaero Yeoungdeungpo-gu For enhanced content portability, Illumina Cell-Free DNA Prep with Enrichment is compatible with user-supplied enrichment oligo nucleotides from Illumina or third-party vendors, including single-stranded DNA (ssDNA) from Integrated DNA Technologies (IDT) and double-stranded DNA (dsDNA) from Twist Bioscience. Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Nano and TruSeq PCR-Free Library Prep Kits. Use the Worklow A template for the HiSeq 2000, HiSeq 2500, MiSeq, or NovaSeq 6000 Systems. Illumina does not recommend pooling libraries across different UD index kit types. February 2019. Workflow A is performed A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Illumina Experiment Manager (IEM) software. xlsx IDT® for Illumina® DNA/RNA UD Indexes Set C, Tagmentation (96 Indexes, 96 Samples) 20042666. Plate layout for the Illumina DNA/RNA UD Indexes (v3) Product page and support page links for illumina . IDT is collaborating the scientific literature (Table 1). Trusight Oncology Tumor. Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20803324 Created Date: 20231108020912Z Adaptors and primers were from either the IDT® for Illumina –TruSeq® DNA UD Indexes (Illumina #20022370), or the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs), NEB #E6440. Purchase Identified the IDT for Illumina Nextera DNA UD index plate layout as IDT for Illumina Nextera DNA UD Indexes Sets A. 37% =96 wells Pass Product details. Type & Size. 20027213) were added in a second PcR, generating amplicons compatible with the NextSeq 2000 System. TruSeq DNA Exome; TruSeq DNA Nano; TruSeq DNA PCR-Free; RNA. the scientific literature (Table 1). IDT for Illumina UMI DNA/RNA UD Indexes Set A for Automation (96 Indexes, 96 IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples) 20040870. Title: eCofA IDT for Illumina UMI DNA Index Anchors for Automation - 20833188 Created Date: 20240227200541Z January 30, 2025 is the last date IDT Illumina DNA/RNA UD Indexes Sets A–D (Catalog nos. and its affiliates ("Illumina"), and are intended solely for the contractual use of its customer in connection with the use of the product(s) described herein and for no other purpose. Leveraging proprietary Adaptase TM technology, this RNA library prep kit enables stranded RNA library construction directly Buy IDT xGen cfDNA & FFPE DNA Library Prep Kit for variant identification of degraded and low-input research samples. Our products are used for applications in the life sciences, oncology PrepareLibrary | Sequence | AnalyzeData public. Revision History. TruSeq DNA Single Indexes. The xGen Broad-Range RNA Library Prep Kit has a fast NGS transcriptomics research workflow with comprehensive transcript coverage and NGS data quality from a broad range of input quantities for Illumina ® sequencing platforms. A 1. Library Prep. The new replacement UD index kits addresses this issue by IDT for Illumina –TruSeq DNA UD Indexes. The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued; the last order date is This section applies to any instruments using XLEAP-SBS Chemistry. IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage. This change in base pair index codes can require adjustments to your sequencing run set up. Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet Illumina Experiment Manager. The steps for amplicon sequencing with the Midnight Panel and the IDT Lotus DNA Library Prep Kit include cDNA synthesis, amplicon generation, fragmentation, end prep, adapter ligation, and a final PCR amplification. As a global company that places high User documentation for the IDT for Illumina DNA/RNA UD Indexes IDT for Illumina Nextera DNA Unique Dual Indexes Sets A–D (384 Indexes, 384 samples) 20027217 8 NovaSeq Xp 4-lane Kit 20021665 2 NovaSeq 6000 S4 Reagent Kit (200 cycles) 20027466 2 QIAamp Viral RNA Mini Kit 20043667 13. TruSeq RNA Single Indexes. When using IDT for Illumina Nextera DNA UD Indexes, use the Library Prep Kit Definition File or Import Sample Sheet appropriate for Illumina Stranded Total RNA Prep: IDT for Illumina – RNA: Amplicon sequencing: AmpliSeq for Illumina Panels: AmpliSeq UD Indexes for Illumina (for ≤ 24 samples) AmpliSeq UD Indexes for Illumina (for > 24 samples) Targeted DNA enrichment: Illumina DNA Prep with Enrichment: IDT for Illumina Nextera UD Indexes Compatible only with IDT for Illumina RNA UD indexes, Ligation. Guidelines for preparing libraries with balanced index combinations for sequencing on Illumina systems. xlsx IDT for Illumina DNA-RNA UD Indexes Set B Tagmentation-label-sheet. The final library is ready for Title: eCofA IDT for Illumina UMI DNA Index Anchors - 20814809 Author: Tabrizy, Rowshan Created Date: 20240110075522Z Index Name i7Basesin Adapter i7Basesfor SampleSheet i5BasesforSampleSheet NovaSeq,MiSeq, HiSeq 2000/2500 i5BasesforSampleSheet iSeq,MiniSeq,NextSeq, HiSeq 3000/4000 We recommend ordering the Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). Two-plex through eight-plex pooling strategies are provided for use of IDT for Illumina TruSeq UD Indexes (96 Indexes, 96 Samples) on the NovaSeq X Series. Introducing the Illumina DNA/RNA UD Indexes. The index adapters sequences are eight bases long. Unique, dual-matched indexes can identify contamination and index hopping events at different levels of multiplexing. Files. Sample Information. Separate purchases of enrichment oligos for each panel, Illumina DNA Prep with or without Enrichment for 16 or 96 samples, and sequencing reagents for your system The IDT for Illumina DNA/RNA UD Indexes (A–D) are a required separate purchase whether you opt for the Combo bundle solution or purchase enrichment and library prep separately. Includes 96, 8 bp indexes sufficient for labeling 96 samples and is compatible with TruSeq Stranded mRNA and TruSeq Stranded Total RNA IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq. These indexes are compatible with the What are the sequences for the IDT and Illumina® indexes: IDT 8, IDT 10, ILMN 8, ILMN 10? These index sequences will be provided once your order has been placed. Trusted customer support. In the 24-indexes plate, the set of 24 indexes is replicated four times. The new replacement UD index kits addresses this issue by Title: eCofA IDT for Illumina UMI DNA Index Anchors - 20896206 Created Date: 20241022175518Z IDT for Illumina RNA UD Indexes Set D, Ligation (96 Indexes, 96 Samples) 20040556. IDT for Illumina - UMI Index Anchors: OverrideCycles,Y151;I8;U10;Y151. IDT for Illumina TruSeq DNA UD Indexes (96 Indexes, 96 Samples), Store at -25°C to -15°C (20022370) Quantity Description; 1: Index Plate: IDT for Illumina TruSeq RNA UD Indexes (96 Indexes, 96 Samples), Store at -25°C to -15°C (20022371) Quantity Description; 1: Index Plate: Support Center / IDT for Illumina TruSeq UD Indexes Support. View All. Product details. General. For comprehensive genomic profiling, pair FUSIONPlex Pan Heme and VARIANTPlex Myeloid panels to interrogate 226 genes for fusions, splicing, exon-skipping, SNVs, indels, CNVs, ITDs and expression A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Illumina Experiment Manager (IEM) software. Cloud Software. Use the IDT for Illumina TruSeq UD Indexes Sample Sheet Templates for IEM to record sequences for IDT for Illumina TruSeq UD Indexes. You can also Illumina DNA Prep, previously known as Nextera DNA Flex, offers a fast, robust, and flexible workflow for preparing normalized, sequencing-ready libraries from a wide range of DNA input User documentation for the IDT for Illumina DNA/RNA UD Indexes Two-plex through eight-plex pooling strategies are provided for use of IDT for Illumina TruSeq UD Indexes (96 Indexes, 96 Samples) on the NovaSeq X Series. RNA Library Prep. XLEAP-SBS chemistry on NextSeq 1000 and The following figures show the plate layouts for IDT for Illumina TruSeq UD Indexes. Fast, Automation-friendly Workflow. The index adapters are 10 bases IDT for Illumina PCR Index Sets 1-4 (384 IDX) includes four PCR plates. Updated section on TruSeq Small RNA indexes to improve clarity and accuracy. Libraries were made from 250 ng of sheared genomic DNA (gDNA) and captured as 1-plex, 4-plex, 8-plex, or 16-plex captures using the xGen AML Cancer Panel (n = 1). Library Prep Kit Kit Definition Files Sample Sheet File; Illumina DNA Prep (formerly Nextera DNA Flex) Set A – 96 Indexes; Sets A-D – 384 Indexes; Illumina Custom Enrichment Panel (108 Samples, 4-Plex) 20025371. Index Adapters. cBot. Illumina dNA Prep Library Prep kits feature innovative, on-bead tagmentation chemistry that supports quick and easy library preparation from various organisms and specimens. NovaSeq 6000. IDT for Illumina DNA/RNA UD Indexes Set D, Tagmentation (96 Indexes, 96 Samples) 20042667. Sample sheet Illumina innovative sequencing and array technologies are fueling groundbreaking advancements in life science research, translational and consumer genomics, and molecular diagnostics. Request exactly the primers you need from the 96 sample indexes available for P5 and P7 Illumina® index primer sequences. The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued; the last order date is PrepareLibrary | Sequence | AnalyzeData public. AmpliSeq. Includes 96, 10 bp indexes sufficient for labeling 96 samples. After subsampling to 85M total reads, the average variant allele frequency for each of the targeted mutations was calculated for each library A 1. Plate layout for the Illumina DNA/RNA UD Indexes (v3) Product page and support page links for You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. Promptly store reagents at the indicated tube At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. Indexes are plated down the plate. Each plate contains 96 unique dual indexes (384 total indexes) for multiplexing next-generation sequencing (NGS) IDT for Illumina –TruSeq DNA and RNA UD Indexes. A A comprehensive offering of high-purity adapters and primers for Illumina sequencing. Illumina Adapter Sequences. Quantity Acronym Description; 4: PK1: Proteinase K: 4: MLB: Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20796316 Created Date: 20231010022701Z IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples) 20040870. Dual-index This method is compatible with Illumina sequencing platforms and only requires ligation reagents within 3-h library preparation, which is similar or even better than the Each kit contains one plate of IDT for Illumina RNA Index Anchors and one plate of IDT for Illumina DNA/RNA UD Indexes Set A, B, C, or D. Sequencing Services. Enrichment Based Library Prep. Access the latest technology today with flexible financing for instruments and reagents. for DNBSEQ Sequencers. Index Read 1 contains both an index and a 9 bp UMI, and is the format for IDT Dual Index Adapters with UMIs. An overview of this workflow shows the steps necessary Product: IDT® for Illumina®- DNA/RNA UD Indexes Set B Product number: 10010227 Illumina part number: 20025080 Batch number: 416058004 Date of manufacture: 2023-NOV-10 Expiration date: 2025-NOV-10 Analysis date: 2023-NOV-15 Test Specification Results Adapter QC by NGS 95% LCI* for Q30 purity ≥90. In the This section provides guidelines for pooling Nextera, Illumina Prep, and Illumina PCR index kits. IDT for Illumina DNA/RNA UD Indexes uses 10 base pair index codes that differ from the Nextera XT and Nextera XT v2 indexes, which use eight base pair index codes. IDT for Illumina UMI DNA/RNA UD Indexes Set B, Ligation (96 Indexes, 96 Samples) 20034702. Adapter sequences can be similar or shared across plates. The IDT for Illumina Unique Dual (UD) index adapters are arranged in the plate to enforce the recommended pairing strategy. Description. Illumina DNA Prep with Enrichment – Tagmentation: Best Practices and Troubleshooting Video. A-tailing is performed before adapter ligation. Instrumentation. Sign in IDT® for Illumina® UMI DNA/RNA UD Indexes Set A, Ligation (96 Indexes, 96 Samples) 20034701. Illumina Korea Hi Investment & Securities building 66 Yeoidaero Yeoungdeungpo-gu IDT for Illumina TruSeq UD Indexes are unique dual (UD) indexes with distinct, unrelated adapters for both index reads. Illumina library prep kits and associated index kits. TruSeq RNA CD Indexes. For more information, refer IDT for Illumina Nextera DNA UD Indexes Files for Illumina Experiment Manager. IDT for Illumina Nextera DNA Unique Dual Indexes Set C (96 Indexes, 96 Samples) 20027215. Includes reagents and Ribo-Zero Plus depletion module for preparing 96 Illumina Stranded mRNA Prep, Ligation (16 samples) 20040532 Illumina Stranded mRNA Prep, Ligation (96 samples) 20040534 Indexes Catalog no. Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20825108 Created Date: 20240129214841Z IDT for Illumina Nextera DNA UD Indexes Set D for NovaSeq, MiSeq, HiSeq 2500 and HiSeq 2000-label-sheet. The anchor sets provide premixed Index 1 (i7) and Index 2 (i5) adapters with the adapters necessary for ligation. Use the Workflow B template for the iSeq 100, HiSeq 3000, HiSeq 4000, HiSeq X, MiniSeq, NextSeq 500, or NextSeq 550 Systems. NextSeq 500/550 For information on which application use, see IDT for Illumina DNA/RNA UD Indexes Compatible Products. IDT for Illumina TruSeq DNA UD Indexes (96 Indexes, 96 Samples), Store at -25°C to -15°C (20022370) Quantity Description; 1: Index Plate: IDT for Illumina TruSeq RNA UD Indexes (96 Indexes, 96 Samples), Store at -25°C to -15°C (20022371) Quantity Description; 1: Index Plate: Compatible only with IDT for Illumina RNA UD indexes, Ligation. 21 l Hybridizationtime—Thetotalamountoftimerequiredtoperformhybridizationstepsinanassay. qPCR. They support DNA and RNA library preps and various plexities. Product: IDT® for Illumina®- DNA/RNA UD Indexes Set B Product number: 10010227 Illumina part number: 20025080 Batch number: 416058004 Date of manufacture: 2023-NOV-10 Expiration date: 2025-NOV-10 Analysis date: 2023-NOV-15 Test Specification Results Adapter QC by NGS 95% LCI* for Q30 purity ≥90. LIMS. The following table shows index adapters (wells) that can be IDT for Illumina PCR Index Sets 1-4 (384 IDX) includes four PCR plates. When using IDT for Illumina Nextera DNA UD Indexes, use the Library Prep Kit Definition File or Import Sample Sheet appropriate for Product: IDT® for Illumina®- DNA/RNA UD Indexes Set A Product number: 10010226 Illumina part number: 20025019 Batch number: 457080585 Date of manufacture: 2023-DEC-01 Expiration date: 2025-DEC-01 Analysis date: 2023-DEC-06 Test Specification Results Adapter QC by NGS 95% LCI* for Q30 purity ≥90. NextSeq 500/550. xlsx Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20796316 Created Date: 20231010022701Z IDT for Illumina – TruSeq RNA UD Indexes v2 (96 Indexes, 96 Samples) 20040871. After fragmentation, the DNA fragments are end User documentation for the IDT for Illumina DNA/RNA UD Indexes Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20815973 Author: Tabrizy, Rowshan Created Date: 20240111015737Z Title: eCofA IDT for Illumina DNA/RNA UD Indexes for Automation - 20828965 Created Date: 20240222160639Z Identified the IDT for Illumina Nextera DNA UD index plate layout as IDT for Illumina Nextera DNA UD Indexes Sets A. Supporting Documents. What's New. xlsx Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20835457 Created Date: 20240304201216Z 2025年1月30日是IDT Illumina DNA/RNA UD Indexes Sets A–D（货号20027213、20027214、20042666和20042667）的最后订购日期，届时IDT for Illumina RNA UD Indexes Sets A–D（货号20040553、20040554、20040555和20040556）将可供订购。 We recommend ordering the Illumina DNA/RNA UD Indexes Sets A-D (20091654, 20091656, 20091658, 20091660). The new replacement UD index kits addresses this issue by IDT for Illumina DNA/RNA UD Indexes, IDT for Illumina Nextera DNA UD Indexes, or Nextera DNA CD Indexes (96 Indexes, 96 Samples) Plates, Store at -25°C to -15°C. Recommended adapters and indexing A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Illumina Experiment Manager (IEM) software. Purchase IDT currently offers three different ready-to-use, universal blocker reagents. You can also access the index sequence files for IDT 8/10 and ILMN 8 in the Custom Adapter Configurator tool. IDT for Illumina Nextera DNA UD Indexes do not include V2 indexes. Title: eCofA IDT for Illumina DNA/RNA UD Indexes for Automation - 20828118 Author: Ramos, Frincess Created Date: 20240215234850Z You can view Archer assays alongside IDT’s xGen™ NGS portfolio to find the best next generation sequencing solution for your lab. The Illumina Lysis Kit is required only for processing blood samples. For comprehensive genomic profiling, pair FUSIONPlex Pan Heme and VARIANTPlex Myeloid panels to interrogate 226 genes for fusions, splicing, exon-skipping, SNVs, indels, CNVs, ITDs and expression Provides pooling guidelines and dual-index strategies for using the 10-base pair IDT for Illumina DNA/RNA UD Indexes or 8-base pair Nextera XT and Nextera XT v2 Indexes with the Nextera XT DNA Library Prep kit. To use Illumina sequencing platforms, the SARS-CoV-2 amplicons generated with the Midnight panel can be fragmented using the xGen DNA Library Prep Kit EZ (with enzymatic DNA fragmentation). Confidently detect more with Archer NGS assay solutions for your solid tumor, blood cancer, immune profiling, and genetic disease research. IDT provides several indexing options with its Custom NGS Adapters. Each plate contains 96 unique dual indexes (384 total indexes) for multiplexing next-generation sequencing (NGS) assays. Promptly store reagents at the indicated tube xGen NGS Adapters & Indexing Primers for Illumina Sequencing. Date. This product was formerly named IDT for Illumina Nextera DNA UD Indexes. The total number of reads from all libraries were summed, and the fraction of the IDT for Illumina DNA/RNA UD Indexes Set C (UDP0193–UDP0288)-25°C to -15°C: IDT for Illumina RNA UD Indexes Set D Ligation (96 Indexes, 96 Samples) (20040556) Quantity Reagent Description; 1: IDT for Illumina RNA Index Anchors-25°C to -15°C: 1: At Illumina, our goal is to apply innovative technologies to the analysis of genetic variation and function, making studies possible that were not even imaginable just a few years ago. Workflow A is performed IDT currently offers three different ready-to-use, universal blocker reagents. IDT does not intend for these products to be used in clinical applications and does not warrant their fitness or suitability for any clinical diagnostic use. NovaSeq X/X Plus. Author: Illumina Created Date: Add sample indexes and P5/P7 sequences with rhAmpSeq Index Primers to create dual-indexed amplicon libraries for Illumina sequencing platforms. Purchase Title: eCofA IDT for Illumina DNA/RNA UD Indexes - 20815973 Author: Tabrizy, Rowshan Created Date: 20240111015737Z IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq. Illumina® Illumina® Reagent format Adaptors and primers were from either the IDT® for Illumina® –TruSeq and the NEBNext Multiplex Oligos for Illumina (96 Unique Dual Index Primer Pairs Set 1-4) were pooled at equimolar concentrations and sequenced on the Illumina NovaSeq® 6000 instrument. Our proprietary RNAse H2-dependent PCR technology generates amplicon libraries for targeted sequencing on Illumina® NGS platforms. Custom Third party Library Prep The IDT for Illumina DNA/RNA UD Indexes Sets A-D (20027213, 20027214,20042666, 20042667) are being discontinued. for Ultima Genomics Sequencers. Index Adapters Pooling Guide. The final library is ready for Title: eCofA IDT for Illumina DNA/RNA UD Indexes for Automation - 20813106 Author: Tabrizy, Rowshan Created Date: 20240110075651Z IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage. We recommend using illumina Purification bead, IDT for Illumina Unique Dual (UD) Indexes (24 or 96) offer increased plexity that enables accurate assignment of reads and efficient use of the flow cell. Index Read 2 is a 10 bp UMI, and is the format for Agilent XT HS. Close. Includes reagents and Ribo-Zero Plus depletion module for preparing 96 IDT for Illumina – TruSeq DNA UD Indexes v2 (96 Indexes, 96 Samples) 20040870. xGen Universal Blockers—10 bp TS Mix is compatible with ligation-based Title: eCofA IDT for Illumina DNA/RNA UD Index Set A - 20827937 Created Date: 20240208224712Z A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Illumina Experiment Manager (IEM) software. MiniSeq. Before preparing libraries, create a sample sheet to record the index adapter combinations and other information about your samples. For example, do not pool Illumina UD Indexes with IDT for Illumina UD Indexes. 1. Table 1. Library Prep Kit Kit Definition Files Sample Sheet File; Illumina DNA Prep (formerly Nextera DNA Flex) Set A – 96 Indexes; Sets A-D – 384 Indexes; This product was formerly named IDT for Illumina Nextera DNA UD Indexes. Refer to Index 2 (i5) Orientation for more information on how to enter i5 bases on the sample sheet This section applies to any instruments using XLEAP-SBS Chemistry. Illumina Experiment Manager. 96 Dual Adapter Index Plate [Optional] Flex Lysis Reagent Kit. Includes reagents and Ribo-Zero Plus depletion module for preparing 96 IDT for Illumina DNA/RNA Unique Dual Index Compatibility on the MiniSeq. Title: eCofA IDT for Illumina DNA/RNA UD Index Set A - 20827937 Created Date: 20240208224712Z A custom product file for use with IDT for Illumina TruSeq UD Indexes and the Illumina Experiment Manager (IEM) software. 1000000041074 v06. Catalog IDs: 20020590, 20020591, 20022370, 20022371. The library prep kits provide depletion, cDNA synthesis, and library prep reagents in a 16- or 96-sample size. . Plate. Documentation, product files, FAQs, and other support resources for the IDT for Illumina UD Indexes. 20040553, 20040554, 20040555, and 20040556) will be available for order. Purchase IDT for Illumina TruSeq Unique Dual (UD) Index kit FAQs for kit configurations and usage. Product Files. Added index plate layouts for AmpliSeq CD Indexes Sets B–D for Illumina. When using IDT for Illumina Nextera DNA UD Indexes, use the Library Prep Kit Definition File or Import Sample Sheet appropriate for When using the obsolete IDT for Illumina DNA/RNA UD Index Sets A, B, C, and D with NextSeq 1000/2000 and NovaSeq X instruments, we have observed a high percentage of 1 base pair mismatch in certain UD index pairs. Dependingontheassay May 26, 2017. uqaxpqi uzyjul fint tfyf cujzt vhcxqp iqkptd hoxmzr gapt owmkf